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Description

The spleen in local and systemic regulation of immunity

Explant outgrowth expansion quantification Whole-well fluorescent images taken using an Incucyte live-cell imaging system (Essen Bioscience) using its 4 objective or phase contrast images taken with a 5/0.12 numerical aperture (NA) dry objective in a Leica wide-field microscope AF6000 were quantified using the Fiji Image J software 92