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Hypoxic mice were administered hypoxic treatment in a closed hypoxic chamber (China Innovation Instrument Co., Ltd, Ningbo, Zhejiang, China), which accurately set the desired hypoxic concentration and pattern

NatureMDPI Long term human safety, pharmacokinetics, optimal dose, systemic exposure and oncologic risk signals are undefined (absence of evidence, not evidence of safety)

Histochemical and immunohistochemical staining Tissue cryosections were fixed for 15 min, incubated with SA--gal staining at 37 C overnight in the dark according to the manufacturers instructions (Solarbio, Beijing, China)

In 2001, Lederberg, a Nobel Prize recipient for work on microbial genetics, defined microbiome as the collective genomes of all the microorganisms inhabiting a specific environment, especially that of the body (Lederberg and McCray, 2001)