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Elevated oxidative stress can be indicated by increased levels of superoxide dismutase, catalase, and glutathione peroxidase, as well as lipid peroxidation, in exposed fish (Newman and Newman, 2014

Analytical size-exclusion chromatography To analyse the oligomeric state of the pHATs, 500 L of each His-tagged pHAT sample ( Ta pHAT and hpHAT) at 1 mg/mL in (25 mM Tris buffer (pH 7.5), 150 mM NaCl, and 0.5 mM TCEP) was injected into a Superdex 200 Increase 10/300 GL column (Cytiva) mounted into an AKTA system (Cytiva AKTA go) equilibrated with the same buffer

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Owerbach D, Rutter WJ, Martial JA, Baxter JD, Shows TB