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Matias I, Diniz LP, Araujo APB, Damico IV, de Moura P, Cabral-Miranda F, Diniz F, Parmeggiani B, de Mello CV, Leite REP, Suemoto CK, Ferreira GC, Kubrusly RCC, Gomes FCA (2023) Age-associated upregulation of glutamate transporters and glutamine synthetase in senescent astrocytes in vitro and in the mouse and human hippocampus

Vitrification and data collection For all importin complexes studied in this paper, typically 2.0 l of importin pre-bound to IBB, xIBB, IBB:Nsp1, Ran-GTP, Ran-GTP:RanBP1, or Ran-GDP:RanBP1 at 2 mg/ml final concentration were applied to a 300-mesh copper Quantifoil R 1.2/1.3 holey carbon grid (EMS) previously glow-discharged negatively for 60 s at 15 mA using an easiGlow (PELCO)

& Liu, Y.-S

Natural compounds like secoemestrin C disrupt ER proteostasis to induce aberrant YAP degradation, while 2-hydroxy nervonic acid provokes ERS-mediated apoptosis through metabolic derivatives [155]