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Description

Horizontal line indicates a value of 0.5 logs, identifying positions 1 to +3 as the most variably selective positions

The LC used was an EASY-nLC 1200 system (Thermo Fisher Scientific, San Jose, CA) harboring a home-pulled emitter integrative 75 m by 35 cm C18 column packed with 1.8 m 120 C18 material (Welch, Shanghai, China)