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coli mutants Bacterial colonies from individual bacterial mutants were streaked on LB agar plates, grown at 37C, and genotyped by PCR using the kanamycin-cassette-specific primers (forward primer 5-CGGTGCCCTGAATGAACTGC-3, reverse primer 5-CAGTCATAGCCGAATAGCCT-3) and genomic primers for individual bacterial mutants, which were designed at starting 100-300 bases upstream of start-codon (forward primer) or downstream of stop codon (reverse primer)

[DOI] [PMC free article] [PubMed] [Google Scholar] 49.Al-Bachari S, Naish JH, Parker GJM, Emsley HCA, Parkes LM

Nat Commun (2019) 10(1):2935

6.4.3 Possibility of Smaller Capsule Sizes Due to SAGs high bioavailability, smaller doses are required to achieve the same efficacy