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B1b cells are able to undergo somatic hypermutation and therefore have a more expanded IgM repertoire that is primarily secreted following stimulation through a TLR or BCR 3,126

Finally, PIP3-induced phosphorylation of PDK1 leads to AKT phosphorylation at Thr308 and the mTOR complex 2 (mTORC2) leads to AKT phosphorylation at Ser473

Hematoxylin-eosin (H&E) and Immunohistochemistry (IHC) For H&E staining, tissue sections were immersed in hematoxylin for 1 min, washed with water for 5 min, and stained with eosin for 20 seconds

Moreover, using conditional knockout mouse models (e.g., chondrocyte-specific Trpv1 / or Slc2a1 / ), the protective effects of CAT on ferroptosis and OA progression in vivo need to be verified, combined with spatial metabolomics to analyze dynamic changes in lipid peroxidation products and antioxidant molecules in joint tissues following CAT intervention