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[DOI] [PMC free article] [PubMed] [Google Scholar] 130.Xiang YY, Xu YY, Bhandari A, Sindan N, Hirachan S, et al

After cleavage with His-TEV to remove the His-MBP tag, the eluted fraction (SNX1-His-SUMO-SNX2a) was further loaded onto a Ni-NTA column and washed with buffer containing 20 mM HEPES (pH 7.4), 200 mM NaCl, and 40 mM imidazole to remove unbound samples