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This stands in contrast to the isolated vitamin B12 treatment, which may not provide the same comprehensive benefits as the combination, thus limiting the potential upregulation of protective proteins and failing to promote the necessary downregulation of others

The high sensitivity (100% for the known carcinogenic and mutagenic NAs tested here) of HepG2 cells supplemented with hamster S9-mix, as compared to PHH and PRH, in principle might make this system a valuable tool for hazard identification and potential follow up of negative/borderline EAT test results to provide further reassurance for the EAT result