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Description

The standard procedure and reagents from the Q5 Site-Directed Mutagenesis kit (NEB) were used to insert mutations, ligate plasmids and transform them into NEB 5 competent cells by heat shock

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The column was maintained at 40 C with a constant flow rate of 400 nL/min

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10.1002/tox.23425 71 MaC.ZhaoY.DingX.GaoB

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doi: 10.2967/jnumed.112.111112 200 WangJ.HeX.MengH.LiY.DmitrievP.TianF.et al

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