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A SYBR Premix Ex Taq (TaKaRa Bio, RR420A) was used to quantify the mRNA levels of related genes

That was a big problem about 20 years ago, due to deterioration in the quality of water sources

doi: 10.1016/j.freeradbiomed.2024.05.013 115 QiuXBiQWuJSunZWangW

Treat the age-decline numbers as historical reference points rather than current clinical benchmarks, no recent peer-reviewed studies have confirmed them [16]