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The phylogenetic tree was constructed using the Neighbor-Joining method with 1000 bootstraps (Saitou and Nei, 1987)

228 YamazakiY.KakizakiS.HoriguchiN.TakagiH.MoriM.NegishiM

Additionally, state regulation requires that no facility shall provide services to minor patients that are intended to change such minors sexual orientation or gender identity, including efforts to change behaviors, gender expressions, or to eliminate or reduce sexual or romantic attractions or feelings towards individuals of the same sex. N.Y

Future studies incorporating prespecified power calculations will be crucial for translating these findings into clinical practice