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Finally, an excess of iron, PUFAs, or peroxides, such as Tert-butyl hydroperoxide (tBOOH) or FINO2, induces ferroptosis (Shah et al., 2018)

2.Lentivirus packaging (1) Take 4 L of lentivirus packaging helper plasmid mixture into a 2 mL centrifuge tube, add 4 g of lentiviral vector plasmid containing the target gene, mix gently, add 40 L of MCE Lentivirus Transfection Reagent and mix gently, incubate for 3 min at room temperature