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At the end of the inoculation, 500 l virus growth medium (high glucose DMEM containing 2% BSA, 2% HEPES (1 M), 1% penicillin-streptomycin and 2 g/ml TPCK-trypsin) was added to each well and the plates were incubated at 37 C under 5% CO 2 to allow the virus-infected MDCK cells to express influenza neuraminidase

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Impact of ROS on T cells The influence of ROS on T cells represents a central paradox in tumor immunology: ROS are indispensable for T-cell activation yet act as major drivers of T-cell exhaustion under chronic exposure