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Description

After rapid mixing, the light absorption A1 and A2 of 30 s and 150 s respectively were measured at 412 nm

10 g of mouse anti-HA IgG (Clone 16B12, Biolegend) was diluted in 200 L PBS-T and bound to 50 L of Invitrogen Protein G Dynabeads for 30 min at RT in a reaction with rotation