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Islets were harvested by handpicking under a microscope and cultured in a cell culture incubator (37C and 5% CO 2 ) in DMEM containing 11.1 or 22.2 mmol/l glucose, 1 mmol/l pyruvate, non-essential amino acids, 100 U/ml penicillin, 100 g/ml streptomycin, 2 mmol/l glutamine, 50 g/ml gentamicin, 10 g/ml fungison and 10% FCS, with or without 1 mmol/l cystine

Quantification of (nonestablished) biofilm properties using ImageJ software

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The residue was washed with 20 mL of pure water and 20 mL of methanol, dried using an aspirator, suspended in pure water and benzene and stirred for 5 min