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Description

2020;6:e04107

Knockout HEK293T cells in a 10-cm dish at approximately 90% confluence were transfected with 7.5 g of pCMV-R8.74psPAX2, 750 ng of pCMV-VSV-G and 7.5 g of plentiCRISPRv2 using TransIT-LT1 (Mirus Bio) following the manufacturers protocol