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Competitive bone marrow transplantation assays were performed using HSCs (Lin c-Kit + Sca1 + CD150 + CD48 ) isolated from 2-year-old Mx1-T3 and Mx1-Cre mice

In clinical settings, the origin of CSCs remains debated: it is unclear whether CSCs arise from NSCs that acquire oncogenic mutations or from differentiated cancer cells that dedifferentiate under selective pressure, such as hypoxia, inflammation, or therapeutic insult

However, this theory is largely derived from clinical observations that aura often precedes the onset of headache, and it must be considered with caution due to significant interspecies variations between human and animal CSD

Additionally, in human cells, we observed fluorescence of transfected CBS in an aggregated profile that is consistent with higher-order oligomerisation and completely dependent upon the integrity of the RD loop 516525