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These discoveries led to the conclusion that a single adaptable cochaperone-scaffold complex delivers nascent iron-sulfur clusters to mammalian respiratory chain complexes IIII (Maio et al

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The infected High Five cells were grown in EX-CELL 420 serum-free medium at 27 C for 72 h before harvesting by centrifugation (15 min, 450 g ) and resuspension of the pelleted cells in the cold lysis buffer containing 50 mM Tris pH 8, 150 mM NaCl, protease inhibitors (10 g ml 1 leupeptin, 20 g ml 1 aprotinin, EDTA-free cOmplete protease inhibitor tablet (Roche, one tablet per 50 ml of buffer) and 1 mM PMSF) and 5 mM DTT