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Enzymes included in the UGT1 and UGT2 subfamily are responsible for the glucuronidation of exo and endogenous compounds, whereas members of the UGT3 and UGT8 subfamilies have their distinct functions (See section ) (Mackenzie et al., 2008)

The supernatant was applied to a Ni-chelating column (Cytiva, Freiburg, Germany) pre-equilibrated in 50 mM sodium phosphate, pH 7.5, 5 M GdmCl, and 10 mM imidazole (equilibration butter)

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