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JAMA ophthalmology

Restorative effects of inulin from codonopsis pilosula on intestinal mucosal immunity, anti-inflammatory activity and gut microbiota of immunosuppressed mice

Li K, Sun Y, Liu S, et al

The amplification protocol used was as follows: initial denaturation at 95C for 3 min, followed by 40 cycles of cyclic denaturation at 94C for 20 s, annealing at 59C for 1min and extension at 72C for 15 s