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Deshwal, S., Forkink, M., Hu, C

E .coli cells can be lysed by French press (though this method is not readily scalable), sonication or detergent-based lysis (many commercial lysis reagents are available)

(Although multiple sclerosis (MS) is classically an autoimmune inflammatory demyelinating disease rather than a primary proteinopathy, we include it here due to overlapping chronic CNS injury and immune dysregulation influenced by the microbiome.) For each disease, specific microbial taxa, metabolites, and pathways implicated in pathogenesis are discussed