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First introduced in clinical practice in the mid-1960s and early 1970s as an indiscriminate biomarker of various liver diseases, cholestasis, and exposure to alcoholic beverages, GGT is presently abandoned in many areas of the world due to cost-benefit issues [24]

Chemiluminescent HRP substrate (ECL Pico or Femto, Life Technologies) was added and membranes exposed to autoradiography film

Taken together, these studies contributed greatly towards the translational application of IgG Fc engineering to alter the Fc:FcRn interaction, and provided further proof of the specificity and pH dependency of the interaction

Consider dose reduction in hepatic failure