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Clustering and gene expression analysis by RNAseqChef in PreNAC and PostNAC in the recurrence and non-recurrence groups

Cell cultures Mouse embryonic fibroblasts (NIH3T3 cells) were obtained from the National Key Laboratory of Genetics (Changsha, China) and cultured in Dulbeccos modified Eagle medium (DMEM)-high glucose (Cat# 11965092, Gibco, USA) containing 10% bovine calf serum (BCS, Sigma, USA) and 1% penicillinstreptomycin solution (Cat# 15140148, Gibco, USA)

Just make sure you have the need for hydration (don't do this prior to running), and that electrolytes are dialed in to prevent hyponatremia

10.1038/nrd1381 Nat