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and FCT (strategic project UID/NEU/04539/2013

The first primer was designed for a part of a promoter sequence (-365 bps) and exon 1 (136 bps) and the second primer for exon 2 (545 bps) of GPX1

Collino, et al

The cells were lysed in lysis buffer [10 mM Tris (pH 7.4), 10 mM KCl, 2 mM MgCl, 1 mM EGTA, 1 mM DTT, 0.05% (v/v) Triton X-100 and 1 protease inhibitor (Pierce)] for 30 min on ice