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Trimethoprim is metabolized in vitro to 11 different metabolites, of which, five are glutathione adducts and six are oxidative metabolites, including the major metabolites, 1- and 3-oxides and the 3- and 4-hydroxy derivatives
The grids were then stained with 2% uranyl acetate (pH 7.0) for 1 min, blotted dry with filter paper, and air-dried for 2 min prior to observation under an HT7800 TEM (Hitachi Limited, Tokyo, Japan)
This, in turn, leads to a decrease in the active form of B6 and likely affects GABA biosynthesis
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