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2.2 Experimental design The Design Expert software (version 13, Stat-Ease Inc., Minneapolis, MN, United States) was employed to create a three-factor, three-level (3) Box-Behnken Design (BBD) with five center points, resulting in 17 experimental runs as depicted in Table 1

The detection of these signatures 24 h after TBI was associated with poorer clinical outcomes at 6 months [31]

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