mitochondrial glutathione Depletion in Diseases The Rockefeller University » How

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The qPCR cycling conditions were as follows: an initial denaturation at 95 C for 15 min, followed by 30 cycles of 95 C for 7 s and 58 C for 10 s, and a final extension step at 95 C for 5 min, then 40 cycles of 95 C for 15 s and 58 C for 30 s

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"Systemic inhibition of IL-6/Stat3 signalling protects against experimental osteoarthritis." Annals of the rheumatic diseases 76.4 (2017): 748-755