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The slides were immersed in 4% paraformaldehyde for 20 min, 1% Triton X-100 for 30 min, and 1% bovine serum albumin for 1 h

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The reaction was measured spectrophotometrically at 340 nm ( Isocitrate Lyase The reaction mixture contained 30 mM imidazole (pH 6.8), 5 mM MgCl 2 , 1 mM EDTA, 4 mM phenylhydrazine and 1 mM D,L-isocitrate, and 0.2 ml of cell extract

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