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Precision-Dosed for Consistency

MAPKs were expressed with an N-terminal His 6 -tag that was cleaved off by the TEV protease after purification with Ni-NTA affinity resin, and then samples were further purified using ion-exchange chromatography (MonoQ, GE Healthcare)

In two independent experiments utilizing different mouse strains, the AF-treated mice had a 59% and 63% decreases in worm burden compared to control mice (Table 5)