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However, there is no gold standard yet for the analysis of barrier function and a clear causeeffect relation has not totally been established, although the role of gut microbiota in these processes is continuously being studied, and significant information are emerging for diagnostic and therapeutic purposes

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This functional unit strictly depends on the integrity of the gut epithelium, supported by junctional proteins, such as tight junctions (TJs), desmosomes, and adherent junctions, which form a physical barrier and connect adjacent epithelial cells, together with the lamina propria