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Thin sections were examined under an electron microscope (JEOL JSM-7001F, JEOL Ltd., Tokyo, Japan), and micrographs were captured by the camera attachment of the microscope

This creates a tent of tissue that ensures the needle enters the subcutaneous layer rather than going too deep

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When to Use Sterile Water Use sterile water for injection when your protocol specifies a preservative-free diluent, or when only a single reconstitution event is needed and the remaining volume can be discarded