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doi: 10.1186/1476-5926-11-1 115 ZhangHZhaoTLiZYanMZhaoHZhuBet al

Cisplatin, vinblastine, and bleomycin combination chemotherapy in metastatic granulosa cell tumor of the ovary

The test was repeated thrice in a row, and the mean value of the holding force was considered

To measure the CRT expressions, after treatment, the cells were washed with cold PBS, and further incubated with anti-CRT polyclonal antibody (diluted 1:100, anti-human antibody for G7, WL1, U87, and U251 cells and anti-mouse antibody for GL261 and G422 cells) at 4 C for 30 min, and stained with Alexa Fluor 647 labeled goat antirabbit IgG (H&L), a highly cross-adsorbed secondary antibody (1:200 dilution), for 30 min at room temperature