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NAC was prepared in phosphate-buffered saline the day prior, balanced for pH (using NaOH), stored under nitrogen, and opened immediately prior to each injection to minimize oxidation

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56 A higher viscosity and mucoadhesive force were observed for chitosan-coated nanoemulsions compared to non-coated nanoemulsions, owing to the well-known enhancing effect of chitosan on the mucoadhesive profile

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