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Description

BMC Plant Biol 21(1):174

Cell pellets were resuspended in Lysis Buffer (20 mM HEPES/KOH pH 7.5, 150 mM KOAc, 5 mM MgCl 2 , 1 mM DTT, 0.5% IGEPAL CA-630 (Sigma Aldrich), protease-inhibitor mix (homemade)) and lyzed in a dounce homogenizer (Wheaton) with ten strokes using the loose pestle