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The campaign gathered over 200 participants from across the country, eventually narrowing the selection down to eight Glow Ambassadors: Khate Mendoza, Erica Calimag, Leiren Lim Canceran, Mikko Abelardo, Ria de Guzman, Nathalie Pascual, Karen Dawn Revolledo, and Rose Mae Daga

Blots were blocked with phosphate-buffered saline with 0.1% Tween 20 and then bound with antibodies for Biotin, after which an enhanced chemiluminescence (ECL) reaction was performed for blot development