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As alternative strategies avoiding animals, we mention the usage of: (i) In vitro cell and tissue culturesroutinely used for the preliminary screening of potential drug toxicity and involves the in vitro growth of animal/human cells outside the body in the laboratory environment

Lin Y-L, Liu Y-K, Tsai N-M, Hsieh J-H, Chen C-H, Lin C-M, Liao K-W (2012) A Lipo-PEG-PEI complex for encapsulating curcumin that enhances its antitumor effects on curcumin-sensitive and curcumin-resistance cells, nanomedicine: nanotechnology

The main barriers at this stage are the lack of standardized laboratory assays and the high cost and limited availability of advanced imaging

Tang, C