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Don't be fooled by imidazole! It absorbs UV280, just like protein on chromatogram it'll be seen! Imidazole is great for purifying His tagged proteins, but it's a pain for when you want to detect and PROTEINS: Structure, Function, and Bioinformatics Protein Science Journal Wiley Online Journal GST Tag Definition & Data Full article: Assessment of glutathione levels in model solution and grape ferments supplemented with glutathione enriched inactive dry yeast preparations using a novel UPLC MS MS method

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