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Short term methionine restriction increases hepatic global DNA methylation in adult but not young male C57BL/6J mice

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Many techniques including precipitation, ultracentrifugation (UC), size-exclusion chromatography (SEC), ultrafiltration (UF), density gradient centrifugation (DGC), affinity isolation, and state-of the-arts microfluidic techniques have been developed and used to isolate and purify bEVs