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The active effects may be short, but detection windows can vary depending on dosage, frequency of use, metabolism, and testing methods

Evaluation of antioxidant capacity of SAC DPPH Free Radical Scavenging Capacity Assay Kit (Nanjing Jiancheng Bioengineering Institute, A153-1-1, Nanjing, China): Prepare the working solution according to the kit instructions, mix with different concentrations of SAC solution, incubate in the dark at 25 for 30 min, centrifuge at 4000 rpm for 5 min, transfer 100L to a cuvette, zero with 80% methanol, and measure absorbance at 517 nm

PLoS ONE 12 , e0171293 (2017)