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Rarely, allergic reactions can occur

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The major obstacle in developing such therapies will be the ubiquitous presence of the UPS in eukaryotes which may require nanomedicine approaches or other tissue-targeting strategies to minimize adverse effects of such therapies

Enrichment of Phosphorylated Proteins Assay For phosphorylated proteins enrichment assay, spinal cords were lysed in a phospho-buffer (20 mM Tris-HCl pH 8.0, 150 mM NaCl, 1 mM Ethylenediamine tetraacetic acid (EDTA), 1 mM Na3VO4, 25 mM NaF, 10 mM Na2P2O7, 50 mM -glycerophosphate, 1% NP-40) containing protease inhibitor cocktail and phosphatase inhibitor cocktail