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Using single-cell RNA sequencing and immunofluorescence, we demonstrate a liver-like cellular repertoire, a higher order cellular complexity, presenting with vascular luminal structures, and a population of resident macrophages: Kupffer cells

Using 1 mg twice weekly instead of 2.55 mg puts you below the level needed for angiogenesis

These animals were housed under a controlled 12:12-hour lightdark cycle and provided unrestricted access to food and water throughout the experimental timeline [2]