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Fully reduced protein was incubated with indicated concentrations of APR-246 for one hour at 37 C and desalted with Amicon Ultra Centrifugal Spinners with 3000 cutoff for 30 min at 14000 g to remove excess compound

10.1159/000159843 24 ChenT.JiangY.WangC.CaiZ.ChenH.ZhuJ.et al (2021)

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