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These supports our previous findings with MB49 and BMDMs ( 3.3 Innate immune pathways involved in melanoma (B16-F10) treatment with BCG In order to determine the role of innate immune pathways related to BCG immunotherapy in vivo , we compared our model in WT ( Figure 1A ) with different KO mice (TLR2 -/- , TLR3 -/- , TLR4 -/- , TLR7 -/- , TLR3/7/9 -/- , Caspase-1/11 -/- , Caspase-11 -/- , IL-1R -/- , cGAS -/- , STING -/- and IFNAR -/- )

Reactive oxygen and nitrogen species increase, and antioxidants (e.g., glutathione, vitamins A and E, superoxide dismutase, and catalase) decrease, affecting nuclear-factor erythroid 2related factor (Nrf2)enzymatic processes

10.1016/j.ultsonch.2017.03.040 Ultrason Sonochem

The sequential SFE + UAE process proved to be efficient in obtaining a lipophilic extract by SFE, rich in volatile compounds, and a hydrophilic extract by UAE, rich in curcuminoids and phenolic compounds, using generally recognized as safe (GRAS) solvents, which is consistent with the principles of green chemistry and the Sustainable Development Goals (SDGs)