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Statistical analyses were done using SPSS version 20 (Chicago, IL, U.S.A.)

10.1016/j.redox.2013.01.007 Redox Biol

The APOX assay was conducted according to the method of Nakano and Asada 44 , by following the decrease in the absorbance at 290 nm as ascorbate disappeared, with modifications for use in a microplate reader 45