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The mechanism is still active in leaner individuals just with proportionally smaller output

Thus, the following two tests can be used to confirm B12 issues on a cellular level: Homocysteine is a valid and reliable intracellular/functional marker of methylation

Regarding the SEV group, B12-upregulated genes had GO annotations related to cytoplasmic translation (tRNA and rRNA processing), adaptive immune response, ncRNA processing, miRNA-mediated gene silencing, proteasome-mediated ubiquitin-dependent protein catabolic process, cobalamin transport, and negative regulation of transcription by RNA polymerase II ( Figure S3 )