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On the other hand, we could not detect any downregulation of microglial homeostatic genes ( p2ry12 and cx3cr1 ), possibly because it was difficult to detect differences in qRT-PCR using bulk samples

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Briefly, cells were seeded in 24 well-plates in high-glucose DMEM containing 10% FBS at an initial density of 4 10 4 cells/well and incubated for 48 h at 37 C in a 5% CO 2 atmosphere