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These were assessed with an HRP-conjugated polymer system (K5007, Dako, Germany) and a Leica DM2000 scanner (Leica Biosystems, Wetzlar, Germany)

Severe deficiency may lead to more radical treatments

Although the NAFLD-like phenotype was observed in mice fed with high-fat-Met and Tyr-deficient diets, Nrf2 accumulation was suppressed where nuclear transport of Nrf2 and fumarate in the liver was reduced (Sano et al., 2021)