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38 In 2020, large-scale studies systematically analyzed the tumor-associated microbiota across cancer types via next-generation sequencing and advanced bioinformatics

Although the rationale behind the use of this medium for human PMNs is that the use of whole blood is essential to the separation, see Polymorphprep Application Sheet (on home page, select Products) for more details, Shibata et al [39] used a PMN-rich suspension from which the erythrocytes had been removed

Representative tissue sections were processed for further histological analysis as described previously[2-7,34]