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Proteolytic activity assay To determine the activity of the proteases against Azocoll and Elastin Congo Red, 2.5 g of each KLIKK protease was diluted in assay buffer (50 mM Tris, pH 8.0, 2.5 mM CaCl 2 , and 0.02% NaN 3 ) to a final volume of 125 l, and then mixed with 125 l of substrate in assay buffer (15 mg ml 1 ), followed by incubation for 2 h (Azocoll) or 16 h (Elastin Congo Red) at 37C with shaking

Adjunctive PRP gel significantly enhances the efficacy of tacrolimus ointment in treating erosive oral lichen planus (EOLP), effectively reducing lesions, alleviating pain, and improving the oral immune microenvironment with a lower recurrence rate (89)

Biochemistry 52, 57605769 10.1021/bi400405p [DOI] [PMC free article] [PubMed] [Google Scholar] Bartkowiak A., Grzelinska E., Bartosz G

Jrup L (2002) Cadmium overload and toxicity